The VDR Monoclonal Antibody (CAB20840) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody is raised in rabbits and is highly specific to VDR in human samples, making it ideal for Western blot applications.The VDR protein plays a crucial role in regulating the effects of Vitamin D in the body, influencing processes such as calcium metabolism, cell growth, and immune function. Dysregulation of VDR has been implicated in various diseases, including cancer, autoimmune disorders, and osteoporosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
VDR Monoclonal Antibody
SKU:
CAB20840
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC51248
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NR1I1, PPP1R163, VDR
Positive Sample:
HeLa, Mouse kidney
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Purification Method
Affinity purification
Gene ID
7421
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VDR Rabbit mAb (CAB20840) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
