The AP2S1 Antibody (CAB12270) is a high-quality antibody developed for reliable detection and analysis of target proteins. One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. RRID AB_2759138 Gene ID 1175 Swiss Prot Synonym AP17; FBH3; HHC3; FBHOk; CLAPS2; AP2S1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AP2S1 Antibody
SKU:
CAB12270
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AP17, FBH3, HHC3, FBHOk, CLAPS2, AP2S1
Positive Sample:
HeLa, LO2, SH-SY5Y, Mouse brain, Mouse kidney, Mouse spleen, Rat brain, Rat spleen
One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. RRID AB_2759138 Gene ID 1175 Swiss Prot Synonym AP17; FBH3; HHC3; FBHOk; CLAPS2; AP2S1
Purification Method:
Affinity purification
Gene ID:
1175
RRID:
AB_2759138
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AP2S1 Rabbit pAb (CAB12270) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
