The APRR7 Polyclonal Antibody (CAB22255) is a high-quality antibody developed for reliable detection and analysis of target proteins. PRR7 and PRR9 are partially redundant essential components of a temperature-sensitive circadian system. CCA1 and LHY had a positive effect on PRR7 expression levels. Acts as transcriptional repressor of CCA1 and LHY.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
APRR7 Polyclonal Antibody
SKU:
CAB22255
Size:
100μL, 20μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Seedling, Inflorescences, Before inflorescence, Rosette leaf
Observed MW:
80kDa
PRR7 and PRR9 are partially redundant essential components of a temperature-sensitive circadian system. CCA1 and LHY had a positive effect on PRR7 expression levels. Acts as transcriptional repressor of CCA1 and LHY.
Purification Method
Affinity purification
Gene ID
831793
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 Rabbit pAb (CAB22255) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
