The ARHGAP25 Antibody (CAB1223) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the ARHGAP25 protein, this antibody enables the detection and analysis of ARHGAP25 expression in different cell types, making it ideal for studies in cell biology and signal transduction research.ARHGAP25, also known as Rho GTPase-activating protein 25, plays a crucial role in regulating cell migration, cytoskeletal dynamics, and cell adhesion through its interactions with Rho GTPases.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ARHGAP25 Antibody
SKU:
CAB1223
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KAIA0053, HEL-S-308, ARHGAP25
Positive Sample:
COS-7, OVCAR3
Cellular Localization:
Phagocytic Cup.
Calculated MW:
73kDa
Observed MW:
70kDa
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).
Purification Method
Affinity purification
Gene ID
9938
RRID
AB_2759106
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARHGAP25 Rabbit pAb (CAB1223) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human kidney using ARHGAP25 Rabbit pAb (CAB1223) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of Jurkat cells using ARHGAP25 Rabbit pAb (CAB1223) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using ARHGAP25 Rabbit pAb (CAB1223) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
