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Anti-PADI2 Antibody (CAB12236)

SKU:
CAB12236
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-PADI2 Antibody
Antibody SKU:CAB12236
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PADI2 (NP_031391.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, HT-29, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PADI2 (NP_031391.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLRE RTVR LQYG SRVE AVYV LGTY LWTD VYSA APAG AQTF SLKH SEHV WVEV VRDG EAEE VATN GKQR WLLS PSTT LRVT MSQA STEA SSDK VTVN YYDE EGSI PIDQ AGLF LTAI EISL DVDA DRDG VVEK NNPK KASW TWGP EGQG AILL VNCD RETP WLPK EDCR DEKV YSKE DLKD MSQM ILRT KGPD RLPA GYEI
Gene ID:11240
Uniprot:Q9Y2J8
Cellular Location:Cytoplasm
Calculated MW:49kDa/75kDa
Observed MW:75kDa
Synonyms:PADI2, PAD-H19, PAD2, PDI2
Background:This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.
UniProt Protein Function:PADI2: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm; cytosol

Molecular Function:estrogen receptor binding; protein-arginine deiminase activity

Biological Process: chromatin-mediated maintenance of transcription; establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; peptidyl-citrulline biosynthetic process from peptidyl-arginine; substantia nigra development

NCBI Summary:This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y2J8
NCBI GenInfo Identifier:7531171
NCBI Gene ID:11240
NCBI Accession:Q9Y2J8.2
UniProt Secondary Accession:Q9Y2J8,Q96DA7, Q9UPN2,
UniProt Related Accession:Q9Y2J8
Molecular Weight:49,310 Da
NCBI Full Name:Protein-arginine deiminase type-2
NCBI Synonym Full Names:peptidyl arginine deiminase 2
NCBI Official Symbol:PADI2  
NCBI Official Synonym Symbols:PAD2; PDI2; PAD-H19  
NCBI Protein Information:protein-arginine deiminase type-2
UniProt Protein Name:Protein-arginine deiminase type-2
UniProt Synonym Protein Names:PAD-H19; Peptidylarginine deiminase II; Protein-arginine deiminase type II
Protein Family:Protein-arginine deiminase
UniProt Gene Name:PADI2  
UniProt Entry Name:PADI2_HUMAN
Anti-PADI2 Antibody (CAB12236)Western blot analysis of extracts of various cell lines, using PADI2 antibody (CAB12236) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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