The RhoGAP Antibody (CAB3587) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, shows high specificity and reactivity towards human samples, making it ideal for use in Western blot applications. By targeting the ARHGAP5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it a valuable asset for studies in cell biology and cancer research.ARHGAP5, also known as Rho GTPase-activating protein 5, plays a crucial role in regulating the activity of Rho GTPases, key molecules involved in controlling cell shape, movement, and proliferation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RhoGAP Antibody
SKU:
CAB3587
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GFI2, p190-B, RhoGAP5, p190BRhoGAP, RhoGAP
Positive Sample:
HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Membrane, Peripheral Membrane Protein.
Calculated MW:
172kDa
Observed MW:
185kDa
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
394
RRID
AB_2765168
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RhoGAP Rabbit pAb (CAB3587) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using RhoGAP Rabbit pAb (CAB3587) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
