The ARL13B Antibody (CAB5200) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in various applications, including Western blotting. Its high reactivity with human samples makes it an ideal choice for studies focused on the role of ARL13B in cellular processes.ARL13B is a small GTPase that localizes to the ciliary membrane and plays a critical role in ciliary structure and function. Mutations in the ARL13B gene have been linked to ciliopathies, a group of genetic disorders characterized by abnormalities in cilia structure and function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARL13B Antibody
SKU:
CAB5200
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JBTS8, ARL2L1, ARL13B
Positive Sample:
SKOV3
Cellular Localization:
Cell Projection, Lipid-Anchor, Cilium Membrane.
Calculated MW:
49kDa
Observed MW:
49kDa
This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
200894
RRID
AB_2766056
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from SKOV3 cells, using ARL13B Rabbit pAb (CAB5200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using ARL13B Rabbit pAb (CAB5200) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
