The ASMT Polyclonal Antibody (CAB6529) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ASMT protein, this antibody enables the detection and analysis of ASMT in different cell types, making it an essential tool for studies in the fields of circadian rhythm regulation, sleep disorders, and neurology.ASMT, also known as acetylserotonin O-methyltransferase, plays a crucial role in the biosynthesis of melatonin, a hormone that regulates sleep-wake cycles and other physiological processes.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ASMT Polyclonal Antibody
SKU:
CAB6529
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASMTY, HIOMT, HIOMTY, ASMT
Positive Sample:
293F, Y79
Cellular Localization:
Cytosol.
Calculated MW:
38kDa
Observed MW:
38kDa
This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland. Alternatively spliced transcript variants have been noted for this gene.
Purification Method
Affinity purification
Gene ID
438
RRID
AB_2767122
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ASMT Rabbit pAb (CAB6529) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using ASMT Rabbit pAb (CAB6529) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
