Aspartate Assay Kit - Information
Assay Genie's Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the aspartate concentration in the sample.
Applications
For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.
Aspartate Assay Kit - Key Features
- Sensitive and accurate. Linear detection range: 2 to 400 aspartate for colorimetric assays and 1 to 50 for fluorimetric assays.
Aspartate Assay Kit - Data Sheet | |
| Kit Includes | Developer: 10 mL AST Enzyme: 240 mL Dye Reagent: 120 mL Cosubstrate: 600 mL ODC Enzyme: 120 mL Aspartate Standard: 400 mL |
| Kit Requires | Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes. |
| Method of Detection | OD570nm, or FL530/585nm |
| Detection Limit | OD, FL: 2, 1 |
| Samples | Plasma, serum, tissue, culture media etc |
| Species | All |
| Protocol Length | 60 min |
| Size | 100 tests |
| Storage | Store all kit components at -20 °C. |
| Shelf Life | 6 months |
More Details
ASPARTATE , a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis.
