The Asymmetric DiMethyl-Histone H3-R8 Antibody (CAB3157) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific for detecting the asymmetric dimethylation of histone H3 at arginine 8. It has been validated for use in various applications, including Western blot and immunohistochemistry, and is particularly reactive with human samples.Histone modifications, such as asymmetric dimethylation, play a crucial role in regulating gene expression and chromatin structure. The detection of this specific modification allows researchers to investigate its involvement in various cellular processes, including transcriptional regulation, DNA repair, and cell differentiation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Asymmetric DiMethyl-Histone H3-R8 Antibody
SKU:
CAB3157
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TARK S
Tested Applications:
WBIHC-PIF/ICCChIPELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2764951
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB3157) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R8 antibody (CAB3157).
Immunohistochemistry analysis of paraffin-embedded Rat testis using Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB3157) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb (CAB3157) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Asymmetric DiMethyl-Histone H3-R8 antibody (CAB3157) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
