The ATG12 Monoclonal Antibody (CAB19610) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blotting and immunofluorescence applications.Autophagy is a crucial cellular process that helps maintain cellular homeostasis by degrading damaged organelles and proteins. Dysregulation of autophagy has been linked to various diseases, including cancer and neurodegenerative disorders. ATG12 is an essential component of the autophagy machinery, playing a critical role in the elongation of the autophagosome membrane.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ATG12 Monoclonal Antibody
SKU:
CAB19610
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0089
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Purification Method
Affinity purification
Gene ID
9140
RRID
AB_2862698
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATG12 Rabbit mAb (CAB19610) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 S.
