The ATG4A Polyclonal Antibody (CAB19844) is a valuable tool for researchers studying autophagy, a cellular process involved in the degradation and recycling of cellular components. This antibody, raised in rabbits, is specifically designed to target the ATG4A protein, a key player in the regulation of autophagy. The ATG4A Polyclonal Antibody is highly reactive with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of ATG4A protein levels in various cell types. Its specificity and sensitivity make it an ideal choice for studies in the fields of cell biology, molecular biology, and cancer research.
ATG4A is known to play a crucial role in the initiation and regulation of autophagy, making it a promising target for therapeutic interventions in diseases associated with dysregulated autophagy, such as neurodegenerative disorders and certain types of cancer. By using the ATG4A Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying autophagy and explore new avenues for the development of targeted therapies.
Product Name:
[KO Validated] ATG4A Rabbit Polyclonal Antibody
SKU:
CAB19844
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
Purification Method:
Affinity purification
Gene ID:
115201
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells, using [KO Validated] ATG4A Rabbit pAb (CAB19844) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
