The ATM Antibody (CAB5908) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, offers high reactivity with human samples and is validated for use in various applications such as Western blot and immunofluorescence.ATM, or Ataxia Telangiectasia Mutated, is a crucial protein involved in sensing DNA damage and activating cell cycle checkpoints and DNA repair mechanisms. Dysregulation of ATM function has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATM Antibody
SKU:
CAB5908
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AT1, ATA, ATC, ATD, ATE, ATDC, TEL1, TELO1, ATM
Positive Sample:
NCI-H460
Cellular Localization:
Cytoplasmic Vesicle, Nucleus.
Calculated MW:
351kDa
Observed MW:
310kDa
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
Purification Method
Affinity purification
Gene ID
472
RRID
AB_2766653
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from H460 cells, using ATM Rabbit pAb (CAB5908) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
