The ATOH1 Monoclonal Antibody (CAB11477) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, offers high specificity and sensitivity in detecting ATOH1 protein in various samples, making it ideal for use in immunofluorescence and immunohistochemistry applications.ATOH1, also known as Math1, is essential for the differentiation of sensory cells and plays a crucial role in regulating cell fate and proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATOH1 Monoclonal Antibody
SKU:
CAB11477
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0609
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATH1, HATH1, DFNA89, MATH-1, bHLHa14, ATOH1
Positive Sample:
HeLa, HCT116, A549
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
45kDa
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in neuron differentiation; positive regulation of neuron differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including generation of neurons; inner ear morphogenesis; and positive regulation of inner ear auditory receptor cell differentiation. Predicted to be part of chromatin. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
474
RRID
AB_2861575
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATOH1 Rabbit mAb (CAB11477) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
