The ATOH1 Antibody (CAB6530) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in neuron differentiation; positive regulation of neuron differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including generation of neurons; inner ear morphogenesis; and positive regulation of inner ear auditory receptor cell differentiation. Predicted to be part of chromatin. Predicted to be active in nucleus. RRID AB_2767123 Gene ID 474 Swiss Prot Synonym ATH1; HATH1; DFNA89; MATH-1; bHLHa14; ATOH1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ATOH1 Antibody
SKU:
CAB6530
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATH1, HATH1, DFNA89, MATH-1, bHLHa14, ATOH1
Positive Sample:
SW620, HepG2, human pacenta, Mouse liver, Mouse heart
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
35kDa
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in neuron differentiation; positive regulation of neuron differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including generation of neurons; inner ear morphogenesis; and positive regulation of inner ear auditory receptor cell differentiation. Predicted to be part of chromatin. Predicted to be active in nucleus. RRID AB_2767123 Gene ID 474 Swiss Prot Synonym ATH1; HATH1; DFNA89; MATH-1; bHLHa14; ATOH1
Purification Method:
Affinity purification
Gene ID:
474
RRID:
AB_2767123
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATOH1 Rabbit pAb (CAB6530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
