The BAG2 Antibody (CAB7763) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it an ideal tool for studying the function of BAG2 in various cellular processes.BAG2 is a member of the BAG family of proteins, which are involved in modulating the activity of chaperone proteins like HSP70. BAG2 has been implicated in processes such as proteasomal degradation, apoptosis, and cell proliferation, making it a key player in cellular homeostasis. The BAG2 Polyclonal Antibody binds specifically to BAG2 protein, allowing for its detection and analysis in cell lysates and tissue samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
BAG2 Antibody
SKU:
CAB7763
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BAG-2, dJ417I1.2, BAG2
Positive Sample:
HeLa, Jurkat, HepG2
Cellular Localization:
Axon, Cytosol, Dendrite, Dendritic Microtubule.
Calculated MW:
24kDa
Observed MW:
24kDa
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Purification Method
Affinity purification
Gene ID
9532
RRID
AB_2768555
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BAG2 Rabbit pAb (CAB7763) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
