Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein
The Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated IL-23/IL-12B&IL-23A function and its implications in health and disease.
This product (SKU: RPCB0660) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 21.7 kDa (IL-23 alpha) , 34.7 kDa (IL-12 beta) with an observed molecular weight of 24 kDa (IL-23 alpha) , 40-50 kDa (IL-12 beta) under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein (RPCB0660), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human IL-23R, hFc Tag with the EC50 of 21.0ng/mL determined by ELISA (QC Test).
