Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein
The Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Biotinylated GARP&Latent TGF beta 1 Complex biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0097) is produced using advanced expression systems and features a N-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 66.6 kDa (GARP), 44.2 kDa (Latent TGF beta 1) with an observed molecular weight of 72-76 kDa (GARP), 13, 45-47 kDa (Latent TGF beta 1) under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein (RPCB0097), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Biotinylated Recombinant Mouse GARP&Latent TGF beta 1 Complex Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.
