The BLZF1 Antibody (CAB6430) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Golgin 45, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and protein trafficking research.Golgin 45 is a key player in maintaining the structural and functional integrity of the Golgi apparatus, a crucial organelle involved in protein processing and secretion. Dysregulation of Golgin 45 has been linked to disrupted Golgi organization and impaired protein transport, which can have serious implications for cellular function and homeostasis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
BLZF1 Antibody
SKU:
CAB6430
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables ubiquitin protein ligase binding activity. Acts upstream of or within Golgi organization and Golgi to plasma membrane protein transport. Located in Golgi apparatus and nucleoplasm. Biomarker of hepatocellular carcinoma.
Purification Method
Affinity purification
Gene ID
8548
RRID
AB_2767032
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BLZF1 Rabbit pAb (CAB6430) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using BLZF1 Rabbit pAb (CAB6430). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
