The CASP8AP2 Antibody (CAB15770) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the Casp8AP2 protein, researchers can accurately detect and analyze its expression in various cell types, making it a valuable asset for studies in immunology, cancer research, and beyond.Casp8AP2, also known as FLIP2, is a key player in the apoptotic pathway, acting as a regulator of cell death and survival. Its involvement in inflammation signaling pathways makes it a promising target for research into diseases such as cancer, where dysregulation of apoptosis can lead to uncontrolled cell growth.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CASP8AP2 Antibody
SKU:
CAB15770
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CED-4, FLASH, RIP25, CASP8AP2
Positive Sample:
U-87MG, K-562
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Pml Body.
Calculated MW:
223kDa
Observed MW:
215kDa
This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
9994
RRID
AB_2763189
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CASP8AP2 Rabbit pAb (CAB15770) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
