The Catalase Antibody (CAB11780) is a high-quality antibody developed for reliable detection and analysis of target proteins. Catalase is an antioxidant enzyme that plays a crucial role in protecting cells from oxidative damage by breaking down hydrogen peroxide into water and oxygen. This antibody, generated in rabbits, is highly specific to human catalase and is validated for use in Western blot and immunohistochemistry applications.With its high sensitivity and specificity, this antibody allows for accurate detection and quantification of catalase in various cell types and tissues. Its ability to target catalase makes it an invaluable tool for researchers interested in studying oxidative stress, cellular metabolism, and the role of antioxidants in health and disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Catalase Antibody
SKU:
CAB11780
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAT, catalase, MGC138422, MGC138424, Catalase
Positive Sample:
HeLa, 293T, HepG2, THP-1, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular Localization:
Peroxisome.
Calculated MW:
60kDa
Observed MW:
60kDa
This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.
Purification Method
Affinity purification
Gene ID
847
RRID
AB_2758751
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Catalase Rabbit pAb (CAB11780) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
