The CDK1 Monoclonal Antibody (CAB11420) is a high-quality antibody developed for reliable detection and analysis of target proteins. CDK1, also known as cyclin-dependent kinase 1, is a key player in cell division and is essential for proper progression through the cell cycle.This antibody, produced in rabbits, is highly specific for CDK1 and has been validated for use in various applications, including Western blot and immunofluorescence. Its high reactivity with human samples makes it a versatile tool for investigating CDK1 expression and activity in different cell types and experimental conditions.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CDK1 Monoclonal Antibody
SKU:
CAB11420
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC50607
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, CDC28A, P34CDC2, CDK1
Positive Sample:
HeLa, MCF7, Jurkat, 293T, Mouse spleen
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
983
RRID
AB_2861564
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDK1 Rabbit mAb (CAB11420) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Mouse spleen, using CDK1 Rabbit mAb (CAB11420) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
