The CENPK Antibody (CAB7627) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding specifically to the CENPK protein, this antibody allows for the detection and analysis of CENPK in various cell types, making it an essential component for studies in cell biology and cancer research.CENPK, also known as centromere protein K, is a key player in ensuring the proper distribution of chromosomes during cell division.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CENPK Antibody
SKU:
CAB7627
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P33, Solt, CENP-K, FKSG14, AF5alpha, CENPK
Positive Sample:
HeLa, SKOV3, 293T
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
32kDa
Observed MW:
37kDa
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).
Purification Method
Affinity purification
Gene ID
64105
RRID
AB_2768142
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CENPK Rabbit pAb (CAB7627) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
