The ACHA4 Antibody (CAB1648) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CHRNA4 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for researchers in the fields of neuroscience and pharmacology.The CHRNA4 protein is a key component of nicotinic acetylcholine receptors, which play a crucial role in neurotransmission and are implicated in various neurological disorders, including addiction, epilepsy, and cognitive dysfunction.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
ACHA4 Antibody
SKU:
CAB1648
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1137
RRID
AB_2763705
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse kidney, using ACHA4 Rabbit pAb (CAB1648) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of L-929 cells using ACHA4 Rabbit pAb (CAB1648) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
