The CHST10 Polyclonal Antibody (CAB4307) is a valuable tool for researchers studying CHST10, a key enzyme involved in the biosynthesis of heparan sulfate, a crucial component of the extracellular matrix. This antibody, developed using rabbit immunization, exhibits high specificity and reactivity with human samples, making it a reliable choice for various experimental applications, including Western blot analysis.CHST10, also known as carbohydrate sulfotransferase 10, plays a critical role in modifying heparan sulfate chains, which are involved in cell signaling, adhesion, and modulation of growth factor activities. Dysregulation of CHST10 has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities.
Therefore, studying the function and regulation of CHST10 can provide valuable insights into these pathologies and potentially lead to the development of targeted therapies.By utilizing the CHST10 Polyclonal Antibody, researchers can accurately detect and analyze CHST10 expression levels in different cell types and tissues, enabling a better understanding of its biological functions and its potential as a therapeutic target. This antibody is a valuable resource for investigations in glycobiology, cancer research, and other fields where heparan sulfate-mediated processes play a role.
Product Name:
CHST10 Rabbit Polyclonal Antibody
SKU:
CAB4307
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-250 of human CHST10 (NP_004845.1).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
42kDa
Observed MW:
42kDa
This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.
Purification Method:
Affinity purification
Gene ID:
9486
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from BxPC-3 cells, using CHST10 Rabbit pAb (CAB4307) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
