The Citrulline Histone H3 R2/R8/R17 Rabbit Polyclonal Antibody (AB10015) is a valuable tool for researchers studying epigenetics and chromatin modification. This antibody, raised in rabbits, specifically targets citrullinated forms of histone H3 at arginine 2, 8, and 17 residues. It has been validated for use in various applications, including Western blot and immunohistochemistry, and shows high reactivity with human samples.Histone citrullination is a epigenetic modification that plays a crucial role in gene regulation and chromatin structure.
The Citrulline Histone H3 R2/R8/R17 antibody allows researchers to detect and analyze citrullinated histone H3, providing insights into the role of this modification in cellular processes such as transcription, DNA repair, and cell differentiation. Its specificity for citrullinated histone H3 makes it a valuable tool for studying epigenetic mechanisms in health and disease, including cancer and autoimmune disorders.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using citrulline-Histone H3-R2/R8/R17 Rabbit pAb (CAB18298) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
