Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059)
The Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using high-quality rabbit monoclonal technology, specifically targets the N-terminal fragment of cleaved Gasdermin-D. It has been validated for use in Western blot and immunofluorescence applications, allowing for precise detection and visualization of cleaved Gasdermin-D in various cell types.Gasdermin-D is a key player in the process of pyroptosis, a programmed cell death pathway that is triggered by inflammatory signals. By targeting the N-terminal fragment of cleaved Gasdermin-D, this antibody enables researchers to investigate the mechanisms underlying pyroptosis and its role in inflammatory responses.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cleaved Gasdermin D (N Terminal) Rabbit mAb
SKU:
CAB24059
Size:
20μL
Reactivity:
Human
Clone Number:
ARC57989
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NFLTD
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:70000 - 1:280000
IF/ICC
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
79792
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (CAB24059) at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 20s.
