The Clathrin heavy chain Antibody (CAB7886) is a high-quality antibody developed for reliable detection and analysis of target proteins. Clathrin is a key protein involved in the formation of coated vesicles, essential for the uptake of nutrients and signaling molecules into cells. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot and immunofluorescence applications.By binding to the CLTC protein, this antibody enables researchers to visualize and quantify clathrin-coated vesicles in various cell types, providing insights into the mechanisms of endocytosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Clathrin heavy chain Antibody
SKU:
CAB7886
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
Purification Method
Affinity purification
Gene ID
1213
RRID
AB_2768963
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Clathrin heavy chain Rabbit pAb (CAB7886) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
