The Collagen X/COL10A1 Antibody (CAB18604) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly reactive with human samples and has been validated for use in applications such as Western blotting.COL10A1 is primarily expressed in hypertrophic chondrocytes, where it plays a key role in the development and maintenance of skeletal tissues. Dysregulation of COL10A1 has been linked to various skeletal disorders, making it an important target for investigation in musculoskeletal research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Collagen X/COL10A1 Antibody
SKU:
CAB18604
Size:
20μL, 100μL
Reactivity:
Human, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:5000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).
Purification Method
Affinity purification
Gene ID
1300
RRID
AB_2862361
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using Collagen X/COL10A1 Rabbit pAb (CAB18604) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Collagen X/COL10A1 Rabbit pAb (CAB18604) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
