The CRTAP Antibody (CAB4428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blotting applications. By binding to the CRTAP protein, this antibody allows for the accurate detection and analysis of CRTAP levels in various cell types.CRTAP, also known as cartilage-associated protein, plays a crucial role in collagen modification and is essential for the proper formation of collagen fibers in bone tissue.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CRTAP Antibody
SKU:
CAB4428
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass.
Purification Method
Affinity purification
Gene ID
10491
RRID
AB_2765670
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SW480 cells, using CRTAP Rabbit pAb (CAB4428) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using CRTAP Rabbit pAb (CAB4428) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using CRTAP Rabbit pAb (CAB4428) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
