The NG2/CSPG4 Monoclonal Antibody (CAB21095) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples and has been validated for use in multiple applications, including immunohistochemistry, immunofluorescence, and flow cytometry.NG2/CSPG4 is a key player in cancer progression, as it is overexpressed in various cancer types and is associated with tumor growth, invasion, and angiogenesis. Its role in promoting cancer cell survival and metastasis makes it an attractive target for developing novel cancer therapies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NG2/CSPG4 Monoclonal Antibody
SKU:
CAB21095
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC52162
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Apical Cell Membrane, Cell Projection, Extracellular Side, Single-Pass Type I Membrane Protein, Lamellipodium Membrane.
Calculated MW:
251kDa
Observed MW:
450kDa
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Purification Method
Affinity purification
Gene ID
1464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A375 cells, using NG2/CSPG4 Rabbit mAb (CAB21095) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
