The Cullin 3 Antibody (CAB1078) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Cullin-3 protein, this antibody enables precise detection and analysis in various cell types, making it an essential reagent for studies in cell biology and protein degradation mechanisms.Cullin-3 is known to play a pivotal role in regulating cellular processes such as cell cycle progression, signal transduction, and protein quality control through targeted protein degradation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 3 Antibody
SKU:
CAB1078
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL-3, PHA2E, NEDAUS, Cullin 3
Positive Sample:
MCF7, Mouse testis, Mouse brain, Mouse spleen, Rat testis
Cellular Localization:
Golgi Apparatus, Nucleus.
Calculated MW:
89kDa
Observed MW:
84kDa
This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
8452
RRID
AB_2758216
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cullin 3 Rabbit pAb (CAB1078) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using Cullin 3 Rabbit pAb (CAB1078). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
