The Cullin 3 Monoclonal Antibody (CAB19623) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an excellent tool for Western blot applications.Cullin-3 plays a crucial role in protein degradation and the regulation of various cellular processes, including cell cycle progression and signaling pathways. By targeting Cullin-3 with this antibody, researchers can investigate its function and interactions in different cell types, offering valuable insights into its role in cellular homeostasis and disease development.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 3 Monoclonal Antibody
SKU:
CAB19623
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0102
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL-3, PHA2E, NEDAUS, Cullin 3
Positive Sample:
HeLa, Jurkat, Mouse brain, Mouse testis, Rat brain
Cellular Localization:
Golgi Apparatus, Nucleus.
Calculated MW:
89kDa
Observed MW:
89kDa
This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
8452
RRID
AB_2862703
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cullin 3 Rabbit mAb (CAB19623) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Cullin 3 Rabbit mAb (CAB19623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Cullin 3 Rabbit mAb (CAB19623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
