The Cullin 4A Antibody (CAB2882) is a high-quality antibody developed for reliable detection and analysis of target proteins. CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 4A Antibody
SKU:
CAB2882
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:100
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL4A, cullin-4A, Cullin 4A
Positive Sample:
K-562, BT-474, HeLa, Mouse testis
Cellular Localization:
Nucleoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
80kDa
CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).
Purification Method
Affinity purification
Gene ID
8451
RRID
AB_2764702
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cullin 4A Rabbit pAb (CAB2882) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Cullin 4A antibody (CAB2882). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (CAB2882) at a dilution of 1:1000.
