The CUL5 Antibody (CAB5369) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Cullin-5 is involved in the regulation of protein degradation through the ubiquitin-proteasome system, governing the stability of numerous target proteins that play roles in various cellular processes. The ability to specifically target and detect Cullin-5 with this antibody allows for precise analysis and characterization in different cell types, making it indispensable for investigations in cell biology, molecular biology, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CUL5 Antibody
SKU:
CAB5369
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CUL-5, VACM1, VACM-1, CUL5
Positive Sample:
Rat heart
Cellular Localization:
Cytosol.
Calculated MW:
91kDa
Observed MW:
91kDa
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within cerebral cortex radially oriented cell migration and radial glia guided migration of Purkinje cell. Located in site of DNA damage. Part of Cul5-RING ubiquitin ligase complex.
Purification Method
Affinity purification
Gene ID
8065
RRID
AB_2766179
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat heart, using CUL5 Rabbit pAb (CAB5369) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
