The CWC22 Antibody (CAB13116) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the CWC22 protein, researchers can efficiently detect and analyze its expression in various cell types, making it an essential component for studies in molecular biology and gene expression regulation.CWC22 is a key player in the spliceosome complex, which is responsible for correctly removing introns from pre-mRNA during transcription.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
CWC22 Antibody
SKU:
CAB13116
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NCM, fSAPb, EIF4GL, CWC22
Positive Sample:
Mouse liver, Mouse testis
Cellular Localization:
Nucleus, Nucleus Speckle.
Calculated MW:
105kDa
Observed MW:
110kDa
Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in cytosol and nuclear speck. Part of U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome; and U2-type precatalytic spliceosome.
Purification Method
Affinity purification
Gene ID
57703
RRID
AB_2759965
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CWC22 Rabbit pAb (CAB13116) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
