The Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) (CABS007) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in IF/ICC, FC applications and has demonstrated reactivity against Rabbit samples.
Product Name:
Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007)
SKU:
CABS007
Size:
100μL, 200μL, 50μL
Reactivity:
Rabbit
Conjugate:
Cy3. Ex:548nm. Em:562nm.
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCFC
Recommended Dilution:
IF/ICC
1:100 - 1:800
FC
1:100 - 1:800
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2769089
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol,pH7.3.
Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb(A12409,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007,dilution 1:100)(Red),ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076,dilution 1:200) (Green)
Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Cy3 Goat Anti-Rabbit IgG (H+L)(CABS007, 1:800) was used as a secondary antibody.
