The Cyclophilin 40 Monoclonal Antibody (CAB5097) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this monoclonal antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications.Cyclophilin-40 is a member of the cyclophilin family of proteins that act as peptidyl-prolyl cis-trans isomerases, playing important roles in various biological processes such as protein folding, signal transduction, and immune response regulation. The specific binding of this antibody to Cyclophilin-40 enables accurate detection and analysis of the protein in different cell types, making it a valuable tool for studies in immunology, cell biology, and protein research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cyclophilin 40 Monoclonal Antibody
SKU:
CAB5097
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1260
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYPD, CYP-40, Cyclophilin 40
Positive Sample:
NIH/3T3, Jurkat, C6, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
41kDa
Observed MW:
41kDa
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A.
Purification Method
Affinity purification
Gene ID
5481
RRID
AB_2863442
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclophilin 40 Rabbit mAb (CAB5097) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Cyclophilin 40 Rabbit mAb (CAB5097) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
