The Cystatin C Monoclonal Antibody (CAB8933) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody has been specifically raised in rabbits and is highly reactive with human samples, making it ideal for use in a variety of research applications.Cystatin C is a cysteine proteinase inhibitor that is primarily produced by nucleated cells and is involved in regulating enzyme activity. It has been shown to be a reliable marker for kidney function, with levels in the blood correlating with glomerular filtration rate. Additionally, Cystatin C has been implicated in various disease processes, including cardiovascular disease, diabetes, and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cystatin C Monoclonal Antibody
SKU:
CAB8933
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1357
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARMD11, HEL-S-2, Cystatin C
Positive Sample:
Human plasma, HeLa, SH-SY5Y, Mouse lung,
Cellular Localization:
Secreted.
Calculated MW:
13kDa
Observed MW:
15kDa
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein.
Purification Method
Affinity purification
Gene ID
1471
RRID
AB_2863631
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cystatin C Rabbit mAb (CAB8933) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Cystatin C Rabbit mAb (CAB8933) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
