The DA (Dopamine) ELISA Kit is specifically designed for the precise measurement of dopamine levels in various biological samples, including serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, guaranteeing consistent and accurate results for research purposes.Dopamine is a key neurotransmitter that plays vital roles in regulating mood, emotions, and cognitive functions. Imbalances in dopamine levels have been implicated in various neurological and psychiatric disorders, such as Parkinson's disease, schizophrenia, and addiction. Therefore, this ELISA kit is essential for studying the role of dopamine in these conditions and exploring potential therapeutic interventions.Overall, the DA (Dopamine) ELISA Kit is a valuable tool for scientists and researchers interested in investigating the physiological and pathological roles of dopamine in the human body. Its reliability and precision make it an indispensable asset in the field of neuroscience and pharmacology.
Product Name:
DA (Dopamine) ELISA Kit
SKU:
UNES00029
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
