The DAP Kinase 1 (DAPK1) Monoclonal Antibody (CAB11459) is a high-quality antibody developed for reliable detection and analysis of target proteins. Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DAP Kinase 1 (DAPK1) Monoclonal Antibody
SKU:
CAB11459
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC0601
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAPK, ROCO3, DAP Kinase 1 (DAPK1)
Positive Sample:
OVCAR3, Mouse lung
Cellular Localization:
Cytoplasm, Cytoskeleton, Cytoskeleton.
Calculated MW:
160kDa
Observed MW:
160kDa
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1612
RRID
AB_2861570
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DAP Kinase 1 (DAPK1) Rabbit mAb (CAB11459) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
