The DDAH2 Monoclonal Antibody (CAB4159) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DDAH2 Monoclonal Antibody
SKU:
CAB4159
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0910
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
G6a, DDAH, NG30, DDAHII, HEL-S-277, DDAH2
Positive Sample:
HepG2, Mouse lung, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular Localization:
Cytoplasm, Mitochondrion.
Calculated MW:
30kDa
Observed MW:
30kDa
This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23564
RRID
AB_2863199
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDAH2 Rabbit mAb (CAB4159) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
