The DENND4A Antibody (CAB16095) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications, allowing for the detection and analysis of DENND4A protein expression in a variety of cell types.DENND4A, also known as a guanine nucleotide exchange factor, plays a crucial role in regulating intracellular transport and membrane dynamics, making it a promising target for further research into cellular mechanisms and disease pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DENND4A Antibody
SKU:
CAB16095
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRLB, MYCPBP, DENND4A
Positive Sample:
HepG2, mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
209kDa
Observed MW:
209kDa
This gene encodes a DENN domain-containing protein that may function as a guanine nucleotide exchange factor that specifically activates ras-related protein Rab-10. This protein also contains a interferon stimulated response element-binding domain and may be involved in regulating the v-myc avian myelocytomatosis viral (MYC) oncogene. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.
Purification Method
Affinity purification
Gene ID
10260
RRID
AB_2763537
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DENND4A Rabbit pAb (CAB16095) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
