The Desmin Monoclonal Antibody (CAB3736) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to desmin in human samples and is validated for use in various applications such as immunohistochemistry and western blotting.Desmin is a key component of the intermediate filaments in muscle cells, providing structural support and helping to maintain the organization of the sarcomeres. Dysregulation of desmin expression or mutation of the desmin gene has been implicated in various muscle disorders, making it a crucial target for research in muscle biology and diseases.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Desmin Monoclonal Antibody
SKU:
CAB3736
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0235
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSM1, CSM2, CDCD3, LGMD1D, LGMD1E, LGMD2R, Desmin
Positive Sample:
C2C12, RD, Mouse lung, Mouse heart, Rat heart
Cellular Localization:
Cell Membrane, Cytoplasm, Sarcolemma.
Calculated MW:
54kDa
Observed MW:
54kDa
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
Purification Method
Affinity purification
Gene ID
1674
RRID
AB_2863132
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Desmin Rabbit mAb (CAB3736) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded mouse heart using Desmin Rabbit mAb (CAB3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
