The Desmoglein 3 Monoclonal Antibody (CAB5227) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly reactive antibody, generated in rabbits, is validated for use in various applications such as immunohistochemistry and Western blotting. It specifically binds to the Desmoglein 3 protein, allowing for accurate detection and analysis in different cell types.Desmoglein 3 is a critical component of desmosomes, which play a crucial role in maintaining tissue integrity and barrier function in the skin and mucous membranes. Dysregulation of Desmoglein 3 has been implicated in various skin diseases, including pemphigus vulgaris, a potentially life-threatening autoimmune disorder.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Desmoglein 3 Monoclonal Antibody
SKU:
CAB5227
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1190
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PVA, ABOLM, CDHF6, Desmoglein 3
Positive Sample:
A-431, Cal-27
Cellular Localization:
Cell Junction, Cell Membrane, Single-Pass Type I Membrane Protein, Desmosome.
Calculated MW:
108kDa
Observed MW:
130kDa
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
Purification Method
Affinity purification
Gene ID
1830
RRID
AB_2863494
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Desmoglein 3 Rabbit mAb (CAB5227) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
