The Dimethyl Uhrf1 (K385) PAB Polyclonal Antibody (CAB16008) is a valuable tool for researchers studying the Uhrf1 protein and its role in epigenetic regulation. This antibody, raised in rabbits, is highly specific and reactive with dimethylated Uhrf1 at lysine 385 in human samples. It has been validated for use in various applications such as Western blotting and immunoprecipitation.Uhrf1 is known to play a crucial role in maintaining DNA methylation patterns and gene silencing, making it a key player in epigenetic regulation.
The dimethylated form of Uhrf1 at lysine 385 has been implicated in specific signaling pathways and interactions, making it a target of interest for those studying chromatin remodeling and gene expression.By using the Dimethyl Uhrf1 (K385) PAB Polyclonal Antibody, researchers can gain insights into the function and regulation of Uhrf1 in various cellular processes. Its high specificity and reactivity make it an essential tool for studies in epigenetics, cancer biology, and other related fields.
Product Name:
MonoMethyl-UHRF1-K385 Rabbit Polyclonal Antibody
SKU:
CAB16008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic monomethylated peptide around K385 of human UHRF1 (NP_001041666.1).
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
Purification Method:
Affinity purification
Gene ID:
29128
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody (CAB16008) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
