The DKK3 Monoclonal Antibody (CAB3892) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed from rabbit monoclonal sources, exhibits high specificity and sensitivity for detecting DKK3 protein in human samples, making it ideal for a variety of research applications including Western blotting.DKK3, also known as Dickkopf-3, is a secreted protein that acts as a tumor suppressor by inhibiting tumor cell growth and metastasis. Its dysregulation has been implicated in various cancers, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DKK3 Monoclonal Antibody
SKU:
CAB3892
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0861
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RIG, CRRL, REIC, DKK3
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Extracellular.
Calculated MW:
38kDa
Observed MW:
60kDa
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
27122
RRID
AB_2863155
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DKK3 Rabbit mAb (CAB3892) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of SH-SY5Y cells using DKK3 Rabbit mAb (CAB3892) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
