The DMTF1 Antibody (CAB17067) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly sensitive and specific for use in various research applications, including Western blotting and immunohistochemistry.DMTF1, also known as cyclin D binding Myb-like transcription factor 1, plays a crucial role in the control of cell cycle progression and cell fate determination. Its functions have been linked to various physiological processes, including embryonic development, tissue regeneration, and oncogenesis. By targeting DMTF1, researchers can gain insights into the molecular mechanisms underlying these processes and potentially discover novel therapeutic targets for cancer and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DMTF1 Antibody
SKU:
CAB17067
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DMP1, DMTF, MRUL, hDMP1, DMTF1
Positive Sample:
HT-29
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
84kDa
Observed MW:
85kDa
This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9988
RRID
AB_2769187
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using DMTF1 Rabbit pAb (CAB17067) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
