The DNAJC15 Monoclonal Antibody (CAB4825) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, exhibits high specificity and sensitivity towards DNAJC15 in various research applications.DNAJC15, also known as heat shock protein 40 (HSP40), is essential for proper protein folding and preventing aggregation under stressful conditions. Dysregulation of DNAJC15 has been linked to various diseases, including neurodegenerative disorders and cancer. Therefore, the study of DNAJC15 is vital for understanding cellular protein homeostasis and developing potential therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNAJC15 Monoclonal Antibody
SKU:
CAB4825
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0249
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Predicted to enable ATPase activator activity. Predicted to be involved in protein import into mitochondrial matrix. Predicted to act upstream of or within several processes, including cellular response to starvation; negative regulation of mitochondrial electron transport, NADH to ubiquinone; and negative regulation of protein-containing complex assembly. Predicted to be located in mitochondrial inner membrane. Predicted to be part of PAM complex, Tim23 associated import motor.
Purification Method
Affinity purification
Gene ID
29103
RRID
AB_2863354
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNAJC15 Rabbit mAb (CAB4825) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of NIH-3T3 cells using DNAJC15 Rabbit mAb (CAB4825) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
