The DOK7 Polyclonal Antibody (PAC023751) is a valuable tool for researchers studying DOK7, a cytoplasmic adapter protein involved in neuromuscular development and function. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in various applications, including Western blot and immunohistochemistry.DOK7 plays a crucial role in the signaling pathways that control the formation and maintenance of neuromuscular junctions, making it a key player in muscle function and development. Dysregulation of DOK7 has been linked to neuromuscular disorders, making it an important target for further investigation in the field of neurobiology and muscle diseases.
By using the DOK7 Polyclonal Antibody, researchers can accurately detect and analyze DOK7 expression in different cell types and tissues, providing valuable insights into its function and potential therapeutic targets for neuromuscular disorders. This antibody is a reliable tool for advancing our understanding of DOK7 biology and its implications in human health.
Synthesized peptide derived from N-terminal of human DOK7.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from mouse brain cells, using DOK7 antibody.
Immunofluorescence analysis of HepG2 cells, using DOK7 antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using DOK7 antibody.
Background:
Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
Synonyms:
Dok-7; downstream of tyrosine kinase 7;
UniProt Protein Function:
DOK7: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK. Interacts with the cytoplasmic part of MUSK. Defects in DOK7 are the cause of congenital myasthenic syndrome type 1B or CMS1B. Three isoforms of the human protein are produced by alternative splicing.
The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
